ABSTRACT
We report a case of Weil's disease manifesting elevation of plasma D-dimer in the acute phase. Later, in the convalescent phase after treatment, the plasma levels of D-dimer returned to normal. Plasma D-dimer can be used as a marker of fibrin formation and degradation. Based on our observations, we conjecture that fibrin formation and fibrinolysis may occur in the acute phase of Weil's disease.
Subject(s)
Acute Disease , Adult , Biomarkers/blood , Fibrin Fibrinogen Degradation Products/analysis , Humans , Male , Weil Disease/bloodABSTRACT
Leptospirosis is a zoonosis caused by Leptospira interrogans. This disease is diagnosed by quantification of specific immunoglobulins in serum by the microagglutination test (MAT). The aims of this research were: a) to compare the protein profiles of 3 clinical isolates of bovine leptospirosis with the reference strain used for the MAT, and b) to identify the immunodomain antigens of the regional isolates through PAGE and immunoblotting techniques of bovine sera from infected, vaccinated and MAT-negative animals. Coomassie-blue stained gels revealed extensive protein similarities between pathogenic and reference strain. Most infected (8/10) and vaccinated animal sera (4/7) showed by immunoblotting a similar reactivity against the proteins from pathogenic leptospires, with a strong band of 25-30 kDa which was not detected in the reference strain. The lack of correlation between MAT and immunoblotting techniques for infected animals could be due either to the infection stage at which the diagnosis was made or to the immunoglobulin isotype involved in the response. Results obtained would confirm the antigenic differences between the 3 isolates and the reference strain.
Subject(s)
Animals , Cattle , Antigenic Variation , Antigens, Bacterial/immunology , Weil Disease/veterinary , Cattle Diseases/microbiology , Leptospira interrogans , Agglutination Tests , Antibody Specificity , Antibodies, Bacterial/blood , Antibodies, Bacterial/immunology , Antigens, Bacterial/isolation & purification , Argentina , Bacterial Vaccines , Blotting, Western , Weil Disease/blood , Weil Disease/microbiology , Cattle Diseases/blood , Electrophoresis, Polyacrylamide Gel , Leptospira interrogans , Bacterial Proteins/immunology , Bacterial Proteins/isolation & purification , Reference Standards , Staining and LabelingABSTRACT
Es una zoonosis de distribución mundial, ocasionada por una espiroqueta. La principal fuente de infección para el hombre lo constituyen los roedores. Penetra al hombre a través de piel y mucosas, y luego de un período de incubación de 7 a 12 días ocurre una fase septicemica y una inmune. Sus manifestaciones clínicas incluyen insuficiencia renal aguda, trastornos hematológicos, hepáticos, respiratorios y del sistema nervioso. En la presente revisión se dicuten aspectos epidemiológicos, etropatogénicos clínicos de diagnóstico y tratamiento de esta patología
Subject(s)
Humans , Weil Disease/blood , Leptospirosis/complications , Leptospirosis/immunology , Sepsis/immunology , Zoonoses/parasitology , VenezuelaABSTRACT
Foram estudados 41 pacientes com leptospirose sob os aspectos referentes ao tempo de doença na admissäo hospitalar, e de acordo com este tempo divididos em cinco grupos. Nestes grupos foram analisados as manifestaçöes clínicas, laboratoriais, sorológicas e evolutivas na leptospirose